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Sigma-Aldrich Ribonuclease A from Bovine Pancreas

Part Number: R6513-10MG

Attribute Specification
Brand Sigma-Aldrich
Manufacturer Merck
Product Type Ribonuclease A enzyme
Biological Source Bovine pancreas
Grade Molecular Biology
Form Lyophilised
Specific Activity ≥70 Kunitz units/mg protein
Molecular Mass Approximately 13.7 kDa
EC Number 3.1.27.5
CAS Number 9001-99-4
EC Inventory Number 232-646-6
MDL Number MFCD00132181
NACRES NA.53
DNase Activity None detected
Nickase Activity None detected
Exonuclease Activity None detected
Endonuclease Activity None detected
Storage Temperature −20°C
Hazard Classification Respiratory sensitisation, Category 1
Signal Word Danger
Hazard Statement H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled

Typical applications:

  • Removal of RNA from plasmid and genomic DNA preparations
  • Removal or hydrolysis of RNA in protein samples
  • RNA sequence analysis
  • RNase protection assays
  • Preparation of cells for flow cytometry
  • Immunoperoxidase and immunofluorescence workflows
  • Post-hybridisation processing of tissue sections

Sigma-Aldrich Ribonuclease A (RNase A) is a molecular-biology-grade, lyophilised endoribonuclease derived from bovine pancreas, with a specific activity of at least 70 Kunitz units per mg of protein. It cleaves single-stranded RNA after pyrimidine nucleotides and is used to remove RNA from plasmid DNA, genomic DNA and protein preparations. DNase, nickase, exonuclease and endonuclease activities are not detected.

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