A ready-to-use 2X master mix for standard-speed, probe-based quantitative PCR applications, including gene expression, genotyping, and miRNA analysis. It contains AmpliTaq Gold™ DNA Polymerase, AmpErase™ UNG, dNTPs with dUTP, premixed ROX™ passive reference dye, and optimized buffer components. Catalog number 4304437 contains one 5 mL tube, sufficient for approximately 200 reactions at a 50 µL reaction volume.
Applied Biosystems™ TaqMan™ Universal PCR Master Mix
Part Number: 4304437
| Attribute | Specification |
|---|---|
| Manufacturer brand | Applied Biosystems™ |
| Manufacturer | Thermo Fisher Scientific |
| Catalog number | 4304437 |
| Product type | Universal PCR master mix |
| Concentration | 2X |
| PCR method | qPCR |
| Detection chemistry | Primer-probe / 5′ nuclease |
| Reaction speed | Standard |
| Polymerase | AmpliTaq Gold™ DNA Polymerase |
| Carryover control | AmpErase™ uracil-DNA glycosylase |
| Passive reference dye | ROX™, premixed |
| Sample types | Genomic DNA and cDNA |
| Applications | Gene expression, genotyping, and miRNA analysis |
| GC-rich PCR performance | High |
| Supplied quantity | 1 × 5 mL |
| Approximate capacity | 200 reactions at 50 µL per reaction |
| Storage | 2–8°C, protected from light |
| Manufacturer unit size | Each |
| Intended use | For Research Use Only; not for diagnostic procedures |
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