Agilent AdvanceBio N-Glycanase-PLUS® (PNGase F), 400 mU

AdvanceBio N-Glycanase-plus (PNGase F), ≥10 U/mL (formerly ProZyme). Enzyme releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. A recombinant form of PNGase F. Includes 5x N-glycanase reaction buffer:100 mM sodium phosphate pH 7.5, 0.1% sodium azide; denaturation solution: 2% SDS, 1 M 2-mercaptoethanol; detergent solution: 15% detergent; 5x N-glycanase Tris reaction buffer: 50 mM Tris-HCl pH 8.0